Journal: Animals : an Open Access Journal from MDPI

Article Title: Proteomic Analysis of Differentially Expressed Plasma Exosome Proteins in Heat-Stressed Holstein cows

doi: 10.3390/ani15152286

Figure Lengend Snippet: Isolation and characterization of plasma exosomes. ( A ) Transmission electron microscopy (TEM) image of plasma exosomes. ( B ) Western blot analysis of exosome protein markers (CD63, CD81, TSG101, and Calnexin C). BMECs denote bovine mammary epithelial cells. ( C , D ) Nanoparticle tracking analysis (NTA) of plasma exosomes.

Article Snippet: The membrane was blocked with 5% skim milk for 1 h and then incubated with primary antibodies (CD81, TSG101, CD63, an Calnexin; Proteintech, Rosemont, IL, USA) overnight at 4 °C.

Techniques: Isolation, Clinical Proteomics, Transmission Assay, Electron Microscopy, Western Blot

Journal: Stem Cells International

Article Title: Small Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Enhanced Proangiogenic Potential of Cardiac Fibroblasts via Angiopoietin-Like 4

doi: 10.1155/2022/3229289

Figure Lengend Snippet: Characterization of hucMSCs, sEVs, and CFs. (a) The surface markers of hucMSCs measured by flow cytometry. (b) Morphology of hucMSCs. Scale bar: 1000 μ m (i) and 500 μ m (ii). (c) Morphology of sEVs observed by TEM. Scale bar: 200 nm. (d) Particle size distribution of sEVs measured by NTA. (e) Brownian motion image of hucMSCs. (f) Protein expression of CD9, TSG101, CD81, and calnexin in hucMSCs and sEVs. (g, h) The expression of α -SMA, vimentin, collagen I, periostin, and CD31 on primary CFs was measured by immunofluorescence (g) and western blotting (h). Scale bar: 50 μ m.

Article Snippet: Next, the PVDF membranes were blocked with 5% milk for 1 h and incubated overnight at 4°C with primary antibodies against CD9 (1 : 1,000; Cell Signaling Technology), TSG101 (1 : 1,000; Boster), CD81 (1 : 800; Proteintech), calnexin (1 : 1,000; Boster), α -SMA (1 : 1,000; Boster), vimentin (1 : 1,000; Cell Signaling Technology), collagen I (1 : 1,000; Boster), periostin (1 : 1,000; Proteintech), CD31 (1 : 1,000; Arigo), Angptl4 (1 : 800; Cell Signaling Technology), cyclin D1 (1 : 800; Cell Signaling Technology), β -catenin (1 : 800; Cell Signaling Technology), and GAPDH (1 : 2,000; Cell Signaling Technology).

Techniques: Flow Cytometry, Expressing, Immunofluorescence, Western Blot